Coding
Part:BBa_K632001:Design
Designed by: Viki Ding Group: iGEM11_Minnesota (2011-09-28)
INP-GFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal XbaI site found at 7
Illegal PstI site found at 2113 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal PstI site found at 2113 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal BamHI site found at 337
Illegal XhoI site found at 1387 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal XbaI site found at 7
Illegal PstI site found at 2113 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal XbaI site found at 7
Illegal PstI site found at 2113
Illegal NgoMIV site found at 747 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part contains a fusion of an existent ice nucleation gene (BBa_K265009) with EGFP.
Source
The INP came from the Standard Registry of Biological Parts (BBa_K265009) and the EGFP was amplified from a plasmid.