Coding

Part:BBa_K632001:Design

Designed by: Viki Ding   Group: iGEM11_Minnesota   (2011-09-28)

INP-GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 7
    Illegal PstI site found at 2113
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 2113
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 337
    Illegal XhoI site found at 1387
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 7
    Illegal PstI site found at 2113
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 7
    Illegal PstI site found at 2113
    Illegal NgoMIV site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains a fusion of an existent ice nucleation gene (BBa_K265009) with EGFP.

Source

The INP came from the Standard Registry of Biological Parts (BBa_K265009) and the EGFP was amplified from a plasmid.

References